» Research Facilities

The research laboratories and core facilities (18,200 sq. ft.) are located on the 2nd floor of 9501 Jeronimo Road in Irvine. The facilities include space for dedicated tissue cultures, NMR, microscopy, structural biology, autoclave, and procedure and environmental rooms.

Formal courses for students on laboratory instrumentation and techniques are taught by the Core Facility Manager. Both basic and advanced analytical instrumentation required for biomedical, pharmaceutical, and biotechnological research are available for shared usage. Facility services are available to private companies on a fee-for-service basis.

Equipment

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NGC Medium-Pressure Chromatography Systems

Bio-Rad NGC Medium-Pressure Chromatography Systems (FPLC) for biomolecules purification.

Learn more about the Bio-Rad NGC Medium-Pressure Chromatography Systems.

Shimadzu Nexera X2 Ultra High Pressure Liquid Chromatography (U HPLC)

The UHPLC is an analytical instrument used to identify and characterize the compounds in a mixture under a very high pressure (19,000 psi.) and to provide fast and higher resolution. Different detectors were included in the upgrade.

Learn more about the Shimadzu Nexera X2 Ultra High Pressure Liquid Chromatography (U HPLC).

BD FACSVerse FLOW CYTOMETER

Flow cytometry is used for cell counting, cell sorting, and detection of biomarkers with the use of laser in the fluidic media containing a cell suspension.

Learn more about the BD FACSVerse Flow Cytometer.

Nikon A1R Confocal Microscope

Nikon A1R confocal microscope system provides high-resolution imaging of up to 4096 x 4096 pixels with a galvano (non-resonant) scanner and increased light detection efficiency realizes high image quality.

Nikon Ti-E Inverted Microscope

The Nikon Ti-E is a fully motorized for automated multi-mode image techniques and acquisition. The Ti offers improved system speed, increased flexibility and efficient multi-mode microscopy as part of a fully-integrated microscope system that is ideal for high-end research and live cell imaging.

Learn more about the Nikon Ti-E Inverted Microscope.

C11000 Touch and CFX96 Touch™ Real-Time PCR Detection System

Bio-Rad CFX96™ Optical Reaction Module converts the C1000 Touch™ Thermal Cycler chassis into the powerful and precise CFX96 Touch Real-Time PCR Detection System.

Learn more about the C11000 Touch and CFX96 Touch Real-Time PCR Detection System.

RF-5301PC Spectrofluorophotometer

Compared to absorbance methods, fluorescence sensitivity is tens to thousands times better - this means that you can analyze nanogram to picogram samples with great results. Fluorescence can be used also to identify a specific molecule in a complex background. When the compound of interest does not exhibit natural fluorescence, functional group-specific probes may be used to label the compound.

Learn more about RF-5301PC Spectrofluorophotometer.

ALPHA FT-IR Spectrometer/Platinum ATR Bruker Alpha FTIR interferometer with platinum ATR is based on Bruker Optics` patented RockSolid™ design. It offers full FT-IR sampling flexibility. ALPHA's Platinum ATR single reflection diamond ATR module is easy to use for routine analysis. A variety of sampling options for temperature controlled measurements and liquid flow through analysis complete the versatility of the Platinum ATR.
Bruker Avance III HDTM 400, High Performance Digital Nuclear Magnetic Resonance (NMR) Spectrometer NMR spectroscopy is used to determine the chemical structures, interaction of molecules, and composition of mixtures. Upgrade includes SampleCASE™ automated sample changer and Prodigy™ ultra-high sensitivity multinuclear Broadband CryoProbe (5 mm sample diameter), with high sensitivity 2H lock channel.
Shimadzu BioSpec-nano Shimadzu BioSpec-nano is a micro-volume UV/VIS spectrophotometer. It offers superior detection limits, making it the perfect instrument for quantitation of DNA, RNA, protein analysis, and photometric measurements. Quantitation can be rapidly and simply carried out with very small samples ranging from 1µL-2µL And, with dedicated functions, such as automated sample mounting and wiping. Learn more about Shimadzu BioSpec-nano.
UV-2600 UV-Vis Spectrophotometer Shimadzu UV-2600 is a compact universal, research-grade UV/VIS spectrophotometer that can be used in a wide range of fields, and easily expanded to suit the measurement objective. By using the optional integrating sphere, the measurement wavelength range of the UV-2600 can be extended to the near-infrared region of 1400 nm. Learn more about the UV-2600 UV-Vis Spectrophotometer.

EVOQ Triple Quadrupole LC-TQ Mass Spectrometer Bruker EVOQ triple quadrupole for liquid chromatography (LC-TQ) is exceptional sensitivity, precision, accuracy, linearity, and a wide dynamic range for your multiple reaction monitoring (MRM) assays. Key application this technology can be used includes food Testing, environmental Analysis, and pharmacokinetics. Learn more about EVOQ Triple Quadrupole LC-TQ Mass Spectrometer.
Impact II™, ESI–QTOF Mass Spectrometer Bruker Impact II™ is UHR-QqTOF (Ultra-High Resolution Qq-Time-Of-Flight) mass spectrometry product line with industry-leading > 50,000 Full-Sensitivity Resolution (FSR). It opens up enhanced analytical performance levels for all applications where trace analysis from complex, high-background matrices is a challenge — such as biomarker research, identification of impurities, or residue screening. Learn more about Impact II, ESI-QTOF Mass Spectrometer.
Shimadzu LCMS 2020 Single Quadrupole Liquid Chromatograph Mass Spectrometer (LC/MS) LCMS is used to separate, detect, characterize, and analyze small molecules, proteins, metabolites, pharmaceuticals, and natural products.
Autoflex Speed MALDI-TOF Mass Spectrometer Bruker Autoflex Speed MALDI-TOF and TOF/TOF technology is easy-to-use; robust systems for both traditional MALDI applications (reliable and detailed protein/peptide characterization, and polymer analysis) as well as cutting edge work (high resolution MALDI Tissue Imaging, glycan analysis. Further improved smartbeam™-II laser technology with up to 2,000 Hz repetition rate is a must for MALDI Tissue Imaging and delivers superior performance for any Proteomics project.

Tribute Peptide Synthesizer

Protein Technologies model Tribute peptide synthesizer is a compact benchtop design with chemical-resistant touchscreen and unique Intellisynth UV-Monitoring and Feedback Control system.

Bruker D8 Venture Gallium Liquid Metal Jet Single Crystal Diffraction

The METALJET for X-ray diffraction delivers the smallest and most intensive X-ray beam of any home lab X-ray source to meet the ever increasing demands of modern structural biology including protein crystallography and Small Angle X-ray Scattering.

Learn more about Bruker D8 Venture Gallium Liquid Metal Jet Single Crystal Diffraction.

BioRad ChemiDioc Imager
BioRad Protein i12 IEF Cell for 2D Electrophoresis
BioRad TC20 Automated Cell Counter
BioRad TransBlot Turbo Gel Transfer System
Thermo ST8 Centrifuge
Thermo Savant SpeedVac Concentrator
Thermo MaxQ Shaker

Analytical Core Facility

The CUSP Analytical Core Facility, established in 2015, is located at Chapman University School of Pharmacy, the first School of Pharmacy in Orange County, California. The facility is equipped with both basic and advanced analytical instrumentations required in biomedical, pharmaceutical, and biotechnological research. The facility provides research and training support to researchers from Chapman University and other academic/non-profit institutions and private companies.

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All first-time equipment users must obtain training from the Core Facility staff on properly operating the equipment. The mandatory instrument operator training is provided at no cost. After completing the training users of the facility have independent access to all technologies located in the facility. Full operator-assisted analysis service is available on an additional fee-for-service basis.

The Core Facility provides training, access to the equipment, and any needed assistance. It does not provide consumable supplies, such as solvents, other chemicals, reagents, standards, culture flasks, media, filters, chromatographic columns, pipettes, tips, tissue paper, etc., which are the responsibility of the users. If used, it will be billed in addition to the instrument usage rate.

All first-time users are required to complete the online registration form approved by the principal investigator and sent by e-mail to the Core Facility Coordinator. Unregistered usage of the Facility is prohibited. The Facility is open during normal business hours (8:30 am to 5:30 pm). After-hour access can be granted when specifically requested and approved by the Facility Coordinator. Summer Undergraduate Research Fellows will generally not have after-hour access without supervision by a trained individual from the same laboratory.

All Chapman Investigators have free access
One-time submission or use: create a Purchase Order
Multiple submissions or use: create a blanket order
A minimum unit rate per hour or per sample will be applied

An invoice will be provided for all facility usage on a monthly basis

Instrument	Chapman (Operator Assisted	Academia/ non-profit**	For profit/ Corporate**
Bruker LC/MS	$65/hr	$100/hr	$200/hr
Bruker MALDI-TOF MS	$65/hr	$100/hr	$200/hr
Shimadzu LC/MS	$65/hr	$100/hr	$200/hr
Shimadzu Protein sequencer*	$400/sample	$400/Sample	$500/Sample
Shimadzu UV/VIS Spectrometer	$65/hr	$20/hr	$40/hr
PCR/ RT-PCR	$65/hr	$25/hr	$50/hr
Analytical HPLC/UPLC	$65/hr	$50/hr	$200/hr
Preparative HPLC	$65/hr	$100/hr	$200/hr
Bio-Rad FPLC	$65/hr	$100/sample	$200/sample
Bio-Rad Imager	$65/hr	$25/gel	$50/gel
BD FACS	$65/hr	$100/hr	$200/hr
Bio-Rad PCR and RT-PCR	$65/hr	$50/hr	$100hr
Multi-mode Plate reader	$65/hr	$25/hr	$50/hr
Bruker FTIR Spectrometer	$65/hr	$25/hr	$100/hr
Centrifugation (High Speed / Ultra)	$65/hr	$50/hr	$100/hr
Cell Culture Facility (Hood)	$65/hr	$100/hr	$200/hr
Cell Culture (Incubator)	$25/day	$25/day	$100/day
Microscope Facility	$65/hr	$50/hr	$100/hr

* Operator assisted service only. Minimum sequence of 10 amino acid residue. Additional sequence billed at $40/residues.

** Rates do not include additional operator-assisted service at $65/hr.

All Core Facility users are required to acknowledge specific services in their scientific meeting abstracts and publications: The research was made possible by the use of the CUSP Research Core Facility, supported by the Office of Associate Dean of Research, Graduate Studies, and Global Affairs

NMR Core Facility

Welcome to the NMR Research Core Facility of the School of Pharmacy at the Chapman University. We offer various services to researchers from Chapman University and external users. We can provide training and advise researchers on how to use NMR spectrometers efficiently. We also can run experiments requested by users.

Fee: Researchers and students of Chapman University have reduced base rates as specified below. Spectrometer.

For outside users, current base rates are $20/proton and $50/carbon experiment (tube and solvent included.

Our facility is equipped with a 400 MHz Bruker NMR spectrometer.

5 mm broadband observe (BBO) probe:
- 1H (outer coil); 15N-31P and 19F (inner coil).
Prodigy broadband observes CryoProbeTM:
- 1H/19F (outer coil); 15N-31P (inner coil).
Z-axis pulsed field gradient capability.
Autosampler for automated analysis of up to 24 samples.

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Chapman researchers can use the spectrometer for educational or research projects without the charge.
Students, assistants, and postdocs, who would like to use the spectrometer, should provide a written request from their PI (Chapman University faculty).
All users should demonstrate sufficient proficiency in operating the spectrometer. User training is available upon request.
All users can analyze their samples with the room-temperature BBO (broadband observation) or Prodigy CryoProbeTM. Please contact Dr. Maslennikov in advance if you need to use the CryoProbe.
Please do not bring any radioactive samples – we will not allow you to analyze them under any circumstances.
All self-service users should provide their own tubes, caps, and NMR-compatible solvents. We recommend Norell 508-UP-7 / Wilmad SP528 or better tubes for quantitative analysis and Norell SVCP-5-178 or better tubes for HTP experiments.
Other rules for sample preparation:
- Use PTFE caps for volatile solvents.
- Do not use broken tubes and/or damaged caps.
- Solvent/sample length in the NMR tube: minimum 4 cm, maximum 5 cm.
- If your tube broke accidentally in the magnet, let us know immediately, and do not try to recover the tube – the probe can be damaged.
If you want to use special tubes, please let us know in advance.
We can provide you with appropriate HTP NMR tubes and caps if you return them clean and undamaged.
The facility can provide necessary equipment for sample preparation (pipette samplers, tips, hand centrifuge for NMR tubes) and temporary storage (+4C, -20C).
If you need any additional equipment to handle your samples, please bring it with you or let us know in advance.

Internal users self-service: Free of charge.

Internal and External users non-self-service (rates per sample): Please contact Dr. Innokentiy "Inno" Maslennikov at maslenni@chapman.edu

In the event you use data collected in our Facility for publication, please include acknowledgment of Chapman University School of Pharmacy NMR Core Facility assistance.

Thank you.

» Research Facilities

Equipment

Chromatography

Cytometers

Microscopes

PCR Detection Systems

Spectrofluorophotometers

Spectrometers

Mass Spectrometers

Synthesizers

X-ray Crystallography

Additional Equipment

Analytical Core Facility

Equipment Training

Consumable Supplies

Request for Access

Equipment Fees

Acknowledgement of Facility

NMR Core Facility

General Rules and Requirements

Current Rates for NMR Spectroscopy Services

Facility Acknowledgement